Journal: Molecules
Article Title: DNA Photocleavage in the Near-Infrared Wavelength Range by 2-Quinolinium Dicarbocyanine Dyes
doi: 10.3390/molecules25122926
Figure Lengend Snippet: ( A ) A representative superimposed fluorescence microscopy image reveals intracellular dye localization (red) in ES-2 ovarian clear cell adenocarcinoma cells after incubation with 0.5 μg/mL of dye 4 for 24 h followed by staining nuclei with Hoechst 33342 (blue). Inset: The enlarged area of one cell within the fluorescence microscopy image. ( B ) ES2 cancer cell viability for: Cells, no treatment; Light, cells exposed to a 694-nm laser (~1.3 W/cm 2 ) for 5 min; ( 4 ), cells incubated with dye 4 (0.5 μg/mL = 0.9 μM) for 24 h under dark conditions; and ( 4 ) + Light, cells incubated with dye 4 (0.5 μg/mL) for 24 h and exposed to a 694-nm laser (1.3 W/cm 2 ) for 5 min. * p < 0.05 when compared with non-treated cells. Error bars represent standard deviation.
Article Snippet: ES2 human clear cell ovarian carcinoma cell line was obtained from ATCC (Manassas, VA, USA).
Techniques: Fluorescence, Microscopy, Incubation, Staining, Standard Deviation